BiosearchTech: BHQ Probes used to study BRCA1 and BRCA2 mutation studies. Read the paper: http://bit.ly/cvmwRW
8/26/2010 11:06:29 AM
Novel Technology for Real-time qPCR
Plexor™ primer technology for real-time, quantitative PCR is a new and novel technique that requires only two primers for sensitive and specific quantitation of amplified DNA. The Plexor Technology is available from Promega Corporation of Madison, Wisconsin, Plexor primer technology takes advantage of the highly specific interaction between two modified nucleotides, isoguanine (iso-dG) and 5'-methylisocytosine (iso-dC) which form a unique base pair when incorporated in double-stranded DNA and pair only with each other.
In reactions using Plexor primers, one PCR primer is synthesized with an iso-dC residue and a fluorescent label at the 5' end. The second PCR primer is unlabeled. Iso-dGTP nucleotides, modified to include a dabcyl quencher, are included in the PCR reaction mix. During the amplification reaction only dabcyl-iso-dGTP can be incorporated at the position complementary to the iso-dC residue. Incorporation of the dabcyl-iso-dGTP in close proximity to the fluorescent label effectively quenches the fluorescent signal.
Plexor Primer Design Software
The simplicity of primer design is a distinct advantage of the Plexor System. The Plexor primer design software is now ready for primer design at the Promega website and is available to design primers for single and multiplex qPCR reactions. The Plexor primer design software selects the appropriate fluorescent labels for single and multiplex PCR or quantitative reverse-transcription PCR (qRT-PCR) based on the optics of many popular real-time instruments.
See also:Dual-labeled BHQ® Probes | BHQplus Probes™ | Molecular Beacons | Scorpion® Primers | Amplifluor® Primers | Dye Calibration Standards | Dyes from Biosearch Technologies
